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Periodontitis is an inflammatory disease that affects the supporting structures of teeth. The inflammatory process is characterized by cell activation, degranulation, and the production of inflammatory mediators, which results in alveolar bone loss. Plectranthus Barbatus (P. barbatus) is used as a medicinal plant. It has been reported that P. barbatus inhibits cyclooxygenase-2 (COX-2) expression and that both COX-2 and 5-lipoxygenase (5-LOX) inhibit the inflammatory response. The purpose of this study is to evaluate the effects of P. barbatus extract on the expression of inducible nitric oxide synthase (iNOS) and 5-LOX, and pro-inflammatory cytokines and chemokines in lipopolysaccharide (LPS)-stimulated human periodontal ligament cells (hPDL). hPDL cells were stimulated with LPS alone or LPS + P. barbatus extract for 24 h. A luciferase reporter assay was performed to examine the activity of nuclear factor-kappa B (NF-κB). Gene expression was determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis. Nitric oxide (NO) levels were measured by the Griess assay. The findings revealed that P. barbatus extract reduced the expression levels of iNOS and 5-LOX, and NO levels in LPS-stimulated hPDL cells. The findings also showed that P. barbatus extract inhibited the expression levels of TNF-α, IL-1β, IL-6, and CXCL1 in LPS-stimulated hPDL cells. In addition, P. barbatus extract decreased the expression levels of the NF-κB subunit, p50, and increased the nuclear expression of the NF-κB inhibitor, IkB-α. These results suggest that P. barbatus may affect the LPS-induced inflammatory reaction in hPDL cells by reducing the NF-κB pathway. Therefore, P. barbatus extract could be a potential therapeutic agent to treat periodontitis.I have a friend who is, of all people, a sexual ass
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