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Reactive oxygen species (ROS) are one of the major cell signalling molecules. Cells produce ROS at an early stage of their life, but they are also generated by oxidants and other stimuli for the purposes of cell signalling and in defence against external agents. The paradox of ROS is that they are quite reactive and important for life, but are also destructive when present at higher concentrations. Cells have evolved several scavenging systems, such as enzyme systems (superoxide dismutase, catalase and peroxidases) and non-enzymatic antioxidant molecules (glutathione, lipoic acid, uric acid, vitamins C and E and polyphenols). The antioxidant potential of these molecules is a critical determinant of the outcome of ROS-induced stress, and it has been proposed that cell death in stress conditions is brought about by the failure of the cellular antioxidant system. Therefore the ability of antioxidant molecules to confer protection from ROS effects is of great interest. Owing to the variety of ROS, their associated molecular mechanisms of action, and the many possible ways by which they may be generated or could interact with molecules in the cell, it has been very difficult to analyze and organize this wealth of information. The present paper is intended to illustrate the complexity of the relationship between antioxidant systems and ROS in the face of oxidative stress and offers a discussion of the regulatory mechanisms through which antioxidants play a protective role.Q:
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