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a fantastica viagem dos zoombinisFunctional polymorphisms in the 5-lipoxygenase gene promoter alter susceptibility to atopic dermatitis.
The importance of the 5-lipoxygenase (5-LO) pathway in allergic inflammation has been established by several lines of evidence. Although a wide distribution of 5-LO protein has been detected in various tissues, the role of the 5-LO gene in the pathogenesis of atopic dermatitis has not been determined. To examine the association between the 5-LO gene and atopic dermatitis. To elucidate functional polymorphisms in the 5-LO gene promoter, the genotype and allele frequencies of restriction fragment length polymorphism (RFLP) and the transcriptional activity were studied in patients with atopic dermatitis (n = 197) and the control population (n = 597). The association study was limited to the major histocompatibility complex (MHC) class III region because of the high degree of linkage disequilibrium in this region. The 5-LO gene was found to be in association with atopic dermatitis. The frequency of the 5-LO allele, allele 2, was significantly increased in patients with atopic dermatitis, with odds ratio of 1.86 (P = 0.024). This association was observed primarily in patients with severe atopic dermatitis (odds ratio, 5.72; P = 0.031). On MHC class III analysis, significant associations of atopy with the 5-LO gene were found in the HLA-DR4-DQ8-positive population. The frequency of the 5-LO promoter allele 2 was significantly elevated in patients with atopic dermatitis, with odds ratio of 2.85 (P = 0.008) in total, with odds ratio of 2.58 (P = 0.021) in HLA-DR4-DQ8-positive population. These findings demonstrate that the 5-LO gene polymorphisms associated with an increased risk of atopy, especially severe atopic dermatitis.
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